Article thumbnail

cycloheximide (Sigma-Aldrich Co)

By Cyclosporin A (sigma-aldrich Co

Abstract

methods Cells were grown in YEPD media [1] at 30°C to the density 106 /ml, spun and resuspended in the same volume of fresh media with or without pheromone. For reactive oxygen species (ROS) visualization cells were incubated on a shaker at 30°C for 80 min. before H2DCF-DA (Molecular Probes, Eugene) was added to 10 µM. After 10 min, cells were photographed under 10x objective lens in DIC and FITC channels

Year: 2015
OAI identifier: oai:CiteSeerX.psu:10.1.1.542.689
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://download.cell.com/curre... (external link)
  • http://download.cell.com/curre... (external link)
  • http://citeseerx.ist.psu.edu/v... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.