methods Cells were grown in YEPD media  at 30°C to the density 106 /ml, spun and resuspended in the same volume of fresh media with or without pheromone. For reactive oxygen species (ROS) visualization cells were incubated on a shaker at 30°C for 80 min. before H2DCF-DA (Molecular Probes, Eugene) was added to 10 µM. After 10 min, cells were photographed under 10x objective lens in DIC and FITC channels
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