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Automated Closed-Vessel System for in Vitro Diagnostics Based on Polymerase Chain Reaction

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Abstract

An automated system for polymerase chain reaction (PCR) amplification and detection combats false-positive results caused by “PCR product carryover. ” The system uses a singlevesselfor both PCR amplification and the subsequent detectionof PCR products,eliminatingthe need to handle PCR products in an open environmentand riskproductcar-ryover. The sample and PCR reagents are introduced into one compartment within the vessel, and amplification occurs as they are thermally cycled. Other compartments contain the reagents for detection of PCR products. Pressure from a roller provides for sequential delivery of the contents of the compartments to a detection area The PCR products are biotinylated at their 5 ’ ends during amplification through the use of biotinylated primers. After delivery to the detection area, they are specifically captured by hybridization with im

Year: 2014
OAI identifier: oai:CiteSeerX.psu:10.1.1.489.6968
Provided by: CiteSeerX
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