Protein fractions were extracted by successive extraction and analysis method in four buckwheat varieties: including Japanese spring buckwheat, Japanese summer buckwheat, Yuqiao No.1 and Yuqiao 6-21. The amino acid of the composition and the mineral content of each protein fraction were also analyzed in this paper. The basis for the data on the experiment was the relationships between protein fractions and amino acids, and the mineral contents of protein fractions, the distribution and proportion of amino acids and the minerals in each protein fraction in the buckwheat kernel, as well as the nutritional value of buckwheat kernels were discussed. The results showed that it has a high amount of soluble protein and a very low amount of prolamin in the buckwheat kernel. Leucine is the first limited amino acid in buckwheat, and buckwheat protein is only a semi-nutritional-protein. The buckwheat kernel is rich in K, Zn in albumin, Ca, Mg, Mn in globulin, Na in prolamin and glutein
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