Article thumbnail

Reports

By 

Abstract

Price, availability, and sequencing depth of today’s high-throughput sequencers make gene expression analysis through whole-transcriptome sequencing via mRNA-Seq very competitive with whole-genome microarrays. High-throughput sequencing circumvents hybridization-associated artifacts of microarrays (1–3) and, unlike tag sequencing approaches such as serial analysis of gene expression (SAGE) (4) or massively parallel signature sequencing (MPSS) (5), it can also provide splice variant information and identify mutations if the sequencing library is constructed from whole transcripts. Multiple platforms are available for high-throughput sequencing based mRNA-Seq gene expression such as the Genome Analyze

Topics: SOLiD (Applied Biosystems, Foster City
Year: 2010
OAI identifier: oai:CiteSeerX.psu:10.1.1.456.6702
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.biotechniques.com/m... (external link)
  • http://www.biotechniques.com/m... (external link)
  • http://citeseerx.ist.psu.edu/v... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.