Reports

Abstract

Price, availability, and sequencing depth of today’s high-throughput sequencers make gene expression analysis through whole-transcriptome sequencing via mRNA-Seq very competitive with whole-genome microarrays. High-throughput sequencing circumvents hybridization-associated artifacts of microarrays (1–3) and, unlike tag sequencing approaches such as serial analysis of gene expression (SAGE) (4) or massively parallel signature sequencing (MPSS) (5), it can also provide splice variant information and identify mutations if the sequencing library is constructed from whole transcripts. Multiple platforms are available for high-throughput sequencing based mRNA-Seq gene expression such as the Genome Analyze