Comparing the healing properties of intra-articular injection of human dental pulp stem cells and cell-free-secretome on induced knee osteoarthritis in male rats

Objective: Osteoarthritis (OA) is a common and painful joint disease with multifactorial causes. Stem cells, due to their high ability to reproduce and differentiate, have created a new horizon in tissue engineering of cartilage and bone. Secretions are one of the new therapies that can be used with stem cells or separately. This study aimed to compare the healing effects of human dental pulp stem cells, cell-free secretome, and human dental pulp mesenchymal stem cells with secretome in the induced OA in male rats. Methods: Dental pulp mesenchymal stem cells were isolated and prepared from human dental pulp. The collagenase type II was injected into the knee of twenty-five male Sprague-Dawley rats, and after 10 weeks, OA was confirmed. Rats were divided into five groups (n = 5): 1) Human dental pulp stem cells plus secretome (HDP+Sec); 2) Human dental pulp stem cells (HDP); 3) Secretome (Sec); 4) Hyalgan as the positive control (Hya); 5) No treatment as the negative control (Ctrl). After 12 weeks since OA was confirmed, the healing process was examined by histopathology and radiology evaluations. Results: Histopathological evaluations, radiological assessments, and matrix indexes in three treatment groups significantly improved compared to the Ctrl and Hya groups. Surface in HDP+Sec was significantly better than the Ctrl group. In radiological evaluations, a significant decrease in OA was observed in the three treatment groups in comparison with the Ctrl groups. There was no significant difference between the treatment groups in any radiological and histopathological evaluations. HDP + Sec group slightly records better results compared to Sec or HDP treatment groups. Conclusion: It was concluded that human dental pulp stem cells and their secretome promote cartilage regeneration due to their cell protective potential as well as matrix degeneration reduction capacity. © 2023 Elsevier Lt

Directed targeting of B-cell maturation antigen-specific CAR T cells by bioinformatic approaches: From in-silico to in-vitro

Aims: Chimeric Antigen Receptor (CAR) T-cell is a breakthrough in cancer immunotherapy. The primary step of successful CAR T cell therapy is designing a specific single-chain fragment variable (scFv). This study aims to verify the designed anti-BCMA (B cell maturation antigen) CAR using bioinformatic techniques with the following experimental evaluations. Main methods: Following the second generation of anti-BCMA CAR designing, the protein structure, function prediction, physicochemical complementarity at the ligand-receptor interface, and biding sites analysis of anti-BCMA CAR construct were confirmed using different modeling and docking server, including Expasy, I-TASSER, HDock, and PyMOL software. To generate CAR T-cells, isolated T cells were transduced. Then, anti-BCMA CAR mRNA and its surface expression were confirmed by real-time –PCR and flow cytometry methods, respectively. To evaluate the surface expression of anti-BCMA CAR, anti-(Fab′)2 and anti-CD8 antibodies were employed. Finally, anti-BCMA CAR T cells were co-cultured with BCMA+/- cell lines to assess the expression of CD69 and CD107a as activation and cytotoxicity markers. Key findings: In-silico results approved the suitable protein folding, perfect orientation, and correct locating of functional domains at the receptor-ligand binding site. The in-vitro results confirmed high expression of scFv (89 ± 1.15% (and CD8α (54 ± 2.88%). The expression of CD69 (91.97 ± 1.7%) and CD107a (92.05 ± 1.29%) were significantly increased, indicating appropriate activation and cytotoxicity. Significance: In-silico studies before experimental assessments are crucial for state-of-art CAR designing. Highly activation and cytotoxicity of anti-BCMA CAR T-cell revealed that our CAR construct methodology would be applicable to define the road map of CAR T cell therapy. © 202

The anticoccidial effect of alcoholic extract of Citrullus colocynthis fruit and Juglans regia peel‌ in experimentally infected domestic chicken

One of the main illnesses within the poultry industry is coccidiosis. Anticoccidial medicines applied in the poultry industry show many shortcomings and new control measures are necessary. The current research aimed to study the effect of extract of Citrullus colocynthis and Juglans regia peel on growth performance, gut bacteria, Haematological, Anticoccidial Index (ACI), and Optimum Anticoccidial Activity (OAA) of coccidiosis-infected domestic chicken. The maximum weight gain was observed in the groups treated with 0.001% and 0.01% C. colocynthis extract. Moreover, 0.01% C. colocynthis extract treatment increased two factors of ACI and OAA by 121.42 and 109, respectively, which were higher than commercial anticoccidial (Sulfaclozine). The extract of C. colocynthis fruit and J. regia peel decreased monocytes and eosinophils haematological factors and increased basophils in birds infected with Eimeria. Both extracts modulated intestinal microbiome haematological factors in birds infected with Eimeria, while J. regia peel extract had better performance than C. colocynthis fruit extract. These results indicate that used C. colocynthis and J. regia extracts have an anti-coccidial effect and the potential to control Eimeria infection. © 2023 Elsevier Lt

Mediating role of coping styles on the relationship between personality types and mental disorders in cardiovascular patients: a cross-sectional study in Iran

BACKGROUND: Many mental problems lead to the occurrence of physical diseases, causing worse consequences of diseases. Despite many studies in the field of personality types and types of mental disorders, this relationship and the mediating role of coping styles in cardiovascular patients are still not well known. Therefore, the present study was conducted to investigate the mediating role of coping styles in the relationship between personality types and mental disorders in cardiovascular patients. METHOD: The present study is a cross-sectional study that was conducted on 114 cardiovascular patients at the heart center in Bushehr, Iran. The sampling method is simple random sampling. Demographic information form, MCMI-III questionnaire, NEO-FFI questionnaire, and Lazarus and Folkman coping styles questionnaire were used to collect data. Data were analyzed using SPSS 22 and Amos 24 software. Descriptive statistics methods (mean, variance and percentage), Pearson correlation, and structural equation model (SEM) were applied to analyze the data. RESULTS: The findings showed that the two variables of personality types and problem-oriented explain 15.2% of the variable of mental disorders, of which 10.7% is related to the variable of personality types and 4.5% is related to the intermediate variable of problem-oriented. Among the personality types, the neurotic personality type has the biggest role (0.632) and has a direct and significant effect on mental disorders. Also, the personality types of extroversion (-0.460), agreeableness (-0.312), and responsibility (-0.986) exert inverse and significant effects on mental disorders. CONCLUSION: The results of the present study showed the frequency of personality disorders and other mental disorders among heart patients. Problem-oriented coping style plays a mediating role between personality types and mental disorders. © 2023. The Author(s)

A Pleurocidin-Like Peptide from Poecilia Mexicana Fish Induces Selective Cytotoxicity in Leukemia Jurkat Cells Through The Apoptosis Pathway

Objective: Some cationic anti-microbial peptides show a wide range of cytotoxic action versus malignant cells, which may lead to developing a novel group of antitumor medications. In the present study, the anticancer activity of pleurocidin-like peptide WF3 isoform X2 (AMP-WF3), from the Poecilia Mexicana fish, against leukemic cell line Jurkat was evaluated, and the cytotoxicity compared with the effects on normal cells, including peripheral blood mononuclear cells (PBMCs) and human dermal fibroblast (HDF) cells. Materials and Methods: In this experimental study, cells were treated with various dosages of AMP-WF3 for 24 hours. Using methyl thiazole tetrazolium salt reduction (MTT test), the effects of the AMP-WF3 on cell viability and toxicity were evaluated. The impact of this peptide on apoptotic pathways was examined using flow cytometry and Annexin V-PI stains. Additionally, the relative expression of the P53, P21, and BCL-2 genes was evaluated using a real-time polymerase chain reaction. Results: The Jurkat cell line was more susceptible to AMP-WF3 cytotoxicity [half-maximal inhibitory concentration (IC50)=50 μM], while normal cells (PBMCs and HDF) were less susceptible. Flow cytometry verified that the apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that of HDF and PBMCs. Peptide-treated Jurkat cells were associated with increased expression of P21, and P53 genes. In contrast, the changes in P21, P53, and BCL-2 genes differed in PBMCs and HDF cells. In HDF cells, simultaneous increase of P21, P53, and BCL-2, and in PBMCs, only the increase of BCL-2 was observed. Conclusion: Our research showed that AMP-WF3 could be developed as a novel treatment agent with minimum side effects for ALL patients. © 2023 Royan Institute (ACECR). All rights reserved

Simultaneous Analysis of Mycotoxins, Potentially Toxic Elements, and Pesticides in Rice: A Health Risk Assessment Study

Rice is a widely consumed food worldwide; however, it can be a source of pollutants, such as potentially toxic elements (PTEs), mycotoxins, and pesticides. Sixty rice samples imported from Pakistan (PAK), India (IND), and Thailand (THAI), as well as domestic Iranian (IRN) rice, were collected from Bushehr, Iran, and investigated for the contamination of PTEs, including arsenic (As), lead (Pb), cadmium (Cd), and nickel (Ni); pesticides, including chlorpyrifos, trichlorfon, diazinon, fenitrothion, and chlorothalonil; mycotoxins, such as aflatoxin B1 (AFB1), zearalenone (ZEN), ochratoxin A (OTA), and deoxynivalenol (DON); and molds. Estimated daily intake (EDI) and hazard quotient (HQ) of pollutants and hazard index (HI) and incremental lifetime cancer risk (ILCR) of rice types for the Iranian adult population were calculated. The content of PTEs in Iranian rice was not higher than Iran’s national standard limits. In contrast, other types of rice (imported) had at least one PTE above the permissible level. OTA content was below the detection limit, and all other mycotoxins were within the allowable range in all rice types. Thai rice was the only group without pesticides. The HI order of rice types was as follows: HIPAK = 2.1 > HIIND = 1.86 > HIIRN = 1.01 > HITHAI = 0.98. As was the biggest contributor to the HI of Iranian and Thai rice, and diazinon in the HI of Pakistani and Indian rice. The calculation of ILCR confirmed that the concentrations of Ni and Pb in Pakistani and Ni and As in Indian, Thai, and Iranian rice were not acceptable in terms of lifetime carcinogenic health risks. © 2023 by the authors

Polycyclic aromatic hydrocarbon (PAH) leachates from post-consumption waterpipe tobacco waste (PWTW) into aquatic environment- a primary study

Post-consumption waterpipe tobacco waste (PWTW) is an unrecognized type of hazardous waste that is produced and released in large quantities into the aquatic environment. It may contain high amounts of various pollutants including PAH, and to date, there has been no research on the potential for contamination by PAH from PWTW leaching into aquatic environments. In this study, the concentrations of PAH via PWTW of fruit-flavored and traditional tobacco leachate into three water types, including distilled water (DW), tap water (TW) and sea water (SW) at different contact times were evaluated. There were significantly higher concentration levels of ƩPAH in waters with leachates from fruit-flavored PWTW than traditional tobacco (P-value<0.05). The concentration levels of ƩPAH in DW, TW and SW at a total contact time of two months ranged from 0.13 to 3.51, 0.12 to 3.63 and 0.11–3.64 μg/L, respectively. Lower molecular weight PAH such as naphthalene (Naph), acenaphthylene (Acen) and fluorine (Flu) were detected in leachates immediately after a short contact time of 15 min. Higher molecular weight PAH including benzo [a]anthracene (BaA), benzo [b]fluoranthene (BbF), benzo [k]fluoranthene (BkF), chrysene (Chr), and benzo [a]pyrene (BaP) were detected after one month contact time, while indeno [1,2,3-cd] pyrene (Indp), benzo [ghi]perylene (BghiP) and dibenz [a,h]anthracene (DahA) were only observed at the contact time measurement of two months. By adding sodium azide as an antimicrobial agent and chemical preservative to SW samples, higher concentrations of PAH including IP, DahA and BghiP were observed. The concentration levels of PAH in water samples after two months contact time were higher than water quality standards provided by the World Health Organization (WHO) and other international organizations. © 2023 Elsevier Lt

Post-synthetic modification of dual-porous UMCM-1-NH2 with palladacycle complex as an effective heterogeneous catalyst in Suzuki and Heck coupling reactions

Herein, the synthesis of a novel functionalized metal-organic-framework (MOF) UMCM-1-NH2-furfural/Pd Nano composite [UMCM-1-NH2-F2C-Pd Nano composite] is reported using the post-synthetic modification of UMCM-1-NH2. The targeted complex was prepared while chemically attaching the furfural – using Schiff base reaction followed by complexation with Pd ions. Afterward, the synthesized solid was applied as a very effective multifunctional catalyst in Suzuki and Heck coupling reactions. This technique which was facile, clean, and economically justified used only 0.01 g of the catalyst during the reaction. The easy setup, nontoxic solvent, enhanced yield, short reaction times, high turnover frequency, and catalyst durability are among the marked benefits of this synthesis method. Overall, the high catalytic activity of UMCM-1-NH2-F2C-Pd Nano composite may be due to the obtained high surface area and the synergistic features created between Lewis acidic Zn nodes and Pd ions. © 2023 Elsevier B.V

How much should you worry about contaminant neutrons in spatially fractionated grid radiation therapy?

Objectives: Telehealth monitoring applications are latency-sensitive. The current fog-based telehealth monitoring models are mainly focused on the role of the fog computing in improving response time and latency. In this paper, we have introduced a new service called “priority queue” in fog layer, which is programmed to prioritize the events sent by different sources in different environments to assist the cloud layer with reducing response time and latency. Material and Methods: We analyzed the performance of the proposed model in a fog-enabled cloud environment with the IFogSim toolkit. To provide a comparison of cloud and fog computing environments, three parameters namely response time, latency, and network usage were used. We used the Pima Indian diabetes dataset to evaluate the model. Result: The fog layer proved to be very effective in improving the response time while handling emergencies using priority queues. The proposed model reduces response time by 25.8%, latency by 36.18%, bandwidth by 28.17%, and network usage time by 41.4% as compared to the cloud. Conclusion: By combining priority queues, and fog computing in this study, the network usage, latency time, bandwidth, and response time were significantly reduced as compared to cloud computing

Reforestation of grey mangroves (Avicennia marina) along the northern coasts of the Persian Gulf

Every ecosystem supports the living organisms by providing direct and indirect benefits and services. Among these areas, mangrove forests are one of the most fertile ecosystems in the intertidal zones. These natural forests are one of the coastal ecosystems that not only provide a source of food for humans and animals, but also play a major role in protecting and stabilizing coastlines and controlling climate. Mangroves are a breeding ground and a safe habitat for a variety of marine vertebrates and invertebrates. This study explains the experienced methods of propagation and foresting of Avicenna marina species. For the first time in the Persian Gulf, we reported a 5-year evaluation of reforestation method of grey mangrove. Reforestation in the highest latitude of natural forests in the Persian Gulf did not have any side effects on growing of Grey mangrove in the selected sites. As a result, increasing the area of Grey mangrove forests in the Persian Gulf improves the ecosystems of the tidal area. Increasing the chances of fishing and improving the economic conditions of the local communities around this bay will be some of the results of mangrove forestation. Furthermore, this is the first report of a simple method to train all the steps of grey mangrove reforestation. © 2022, The Author(s), under exclusive licence to Springer Nature B.V