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    YOUTH NAVIGATING PRECARITY THROUGH CLIMATE ACTION: CASE OF MATHARE

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    This study explores the environmental organizing of the youth in Mathare and its potential to transcend hustle into climate activism. It examines how the intervention of global climate actors and their agendas influence the youth's work. By building an empirical case through the stories and evolution of the youth, using observer participation and participant observation, the research analyzes potential pathways and possibilities for Mathare’s youth groups. Embedded in the discourse of hustle and climate adaptation in informal settlements, this paper argues that the youth's work is transitioning from opportunism to visionary practice. As the youth navigate their precarious employment situations and struggle to find identity and community through hustling, the paper explores their potential to expand their vision and scope, and to collectivize towards climate adaptation, is enabled by opportunities presented by global climate efforts. Finally, the paper invites further research on the potential of youth activism to contribute to effective climate adaptation

    Strategic Communication Introduction

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    N/AOverview of the Strategic Communication ToolkitN/

    Reducing Maple Sap Spoilage

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    Maple sap is a perishable product that will spoil when stored improperly or for extended periods of time. As it exits the tree, it is contaminated with bacteria, yeast, and molds, which can spoil the sap by consuming its resources (e.g. sugars) or by releasing byproducts (e.g. enzymes, acids, aromas) resulting in color, texture, flavor and odor changes in the sap. Flavors and textures developed in spoiled sap can persist in maple syrup and other value-added products; proper care of maple sap is essential to produce quality products.Funding for project was made possible by a grant agreement from the U.S. Department of Agriculture (USDA) Agricultural Marketing Service. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA

    Vector-Borne Disease Workforce Training & Development Needs Assessment

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    A primary goal of the Northeast Regional Center for Excellence in Vector-Borne Diseases: Teaching & Evaluation Center (NEVBD-TEC) is to offer professional development opportunities for the public health entomology workforce. In spring 2024, NEVBD-TEC conducted an online needs assessment to understand the training needs for vector-borne disease (VBD) and public health professionals working in public agencies. The assessment focused on those involved in the surveillance and control of ticks, mosquitoes, and their associated diseases in the northeastern United States. This report provides a summary of the outcomes from this training needs assessment.This work was supported through Cooperative Agreement U50CK000633 between Cornell University and the Centers for Disease Control and Prevention. This content is solely the responsibility of the authors and does not necessarily represent the official views of the Centers for Disease Control and Prevention

    Supplemental Materials: The most effective strategy for verifying the absence of sanitizer contamination in milk depends on the sanitizer type used in fluid milk processing facilities

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    Data in support of: The most effective strategy for verifying the absence of sanitizer contamination in milk depends on the sanitizer type used in fluid milk processing facilitiesThis project was supported by the New York State Milk Promotion Advisory Board (Award number C013994) through the New York State Department of Agriculture and Markets (Albany, NY)

    Evaluating a selective therapy approach to antimicrobial treatment of high-risk calves at arrival to a male dairy calf rearing facility on future health, growth and antimicrobial use: A group randomized controlled trial [Supplement]

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    Bovine Practitioner 2025 Supplemental Materials TvK; includes two figures for a permanent link to be published in the next Bovine PractitionerSupplemental materials for Bovine Practitioner articl

    Characterizing the Unique Reliance on Translesion Synthesis in Embryonic Stem Cells in Response to ssDNA Accumulation

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    All replicating cells face an innate amount of replication stress. Stem cells, due to their role as progenitors, must also utilize efficient and high-fidelity pathways to minimize mutagenesis or delays in replication. Embryonic stem cells (ESCs) are rapidly proliferating stem cells isolated from an early embryo and have previously been shown to lack cell cycle checkpoints as well as accumulate much higher amounts of single-stranded (ssDNA) gaps. These gaps are thought to arise from PrimPol restarting stalled forks by repriming downstream from sites of damage, leaving ssDNA that must be filled in post-replicatively by translesion synthesis polymerases Polζ and Rev1. In this study, we utilized a flow cytometry assay that quantifies ssDNA accumulation by BrdU incorporation. Using this assay on cells treated with translesion synthesis (TLS) inhibitor JH-RE-06, we demonstrate the unique reliance of ESCs on TLS polymerase Polζ for ssDNA gap filling in G1 and S phases. Applying this assay revealed differential utilization of TLS in other cell types. Using immortalized mouse embryonic fibroblasts (MEFs), we demonstrate differentiation causes less reliance on TLS, implying reduced ssDNA stress and utilization of other pathways for repair. Through these experiments, we reveal another direction to understand DNA damage response pathways in vivo. We corroborate evidence that reliance on TLS is vital for ESCs. TLS is historically regarded as a low-fidelity pathway, but because ESCs have a much lower mutation rate, we propose that reliance on TLS, specifically Polζ-dependent repair, is in fact a high-fidelity response pathway that is essential for resolving ssDNA gaps

    Research Data Curation Service: 2024 Summary

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    Please cite as: Wright, Sarah J., Lencia Beltran, Gabriella Evergreen, Wendy Kozlowski. (2025) Research Data Curation Service: 2024 Summary [report] Cornell University eCommons Repository. https://hdl.handle.net/1813/116236This 2024 report provides annual highlights of Cornell University Library’s data curation service as well as the number of datasets curated over the past six years, levels of curation performed since we began tracking that in 2021, and a breakdown of inquiries by college. The library’s Research Data and Open Scholarship (RDOS) unit, which was established in fall of 2023, provides data curation as one of our core services. CUL has been offering file-level curation of data and code as an institutional member of the Data Curation Network since 2016

    Identification and Characterization of Rad54 Hypomorphs

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    The maintenance of genomic stability is critical for the proper functioning of all organisms. Various DNA repair processes are involved in safeguarding the integrity of the genome, including homologous recombination (HR), a process that repairs double-stranded breaks in the DNA. The DNA motor protein Rad54 is a highly conserved DNA translocase that is crucial for HR function. In the human context, mutations in its homolog, Rad54L, are known to be associated with many cancer types. This study utilized the model system of Saccharomyces cerevisiae to examine the effects of mutations in Rad54 on important cellular phenotypes. rad54R272Q and rad54R272A demonstrated significant growth defects in response to DNA damage, exhibiting a gradient of activity when compared to the WT. Intriguingly, diploid rad54R272A/rad54R272A displayed a growth defect even under normal conditions. Additionally, the study demonstrated no significant difference in template switching frequencies between the WT and mutants. Together, these results provide greater insights into Rad54’s function in HR and illuminate how mutations within this protein can lead to genome instability, which can contribute to cancer development in humans

    Bottled Maple Sap

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    Producing maple syrup (66.0 to 68.9 °Brix) from maple sap with approximately 2 °Brix requires extensive energy and labor. A lower input option is to market maple sap as a commercial beverage for consumers. On average, about 44 gallons of sap are required to produce one gallon of maple syrup. One gallon of syrup has a gross value of about 120ifsoldin16ozretailcontainers.Ifinsteadofbeingprocessedintosyrup,themaplesapwasprocessedinto16ozbottlesofdrinkablesapsoldat120 if sold in 16 oz retail containers. If instead of being processed into syrup, the maple sap was processed into 16 oz bottles of drinkable sap sold at 3 each, $1,056 of revenue could be generated from the same 44 gallons of sap; this represents a 780% increase in sales revenue. Maple sap is harvested in eastern North America during thawing temperatures from January to April each year. For maple syrup production, sap is typically processed into syrup soon after harvest as sap is a perishable product susceptible to bacteria, yeast, and mold contamination across the season. To preserve maple sap quality, care must be taken to ensure it has not spoiled or interacted with any potential contaminants, including lead and sanitizer residues. Once maple sap is harvested, it can be processed and packaged using multiple techniques, each of which ensure the product maintains quality and safety for consumers. This article reviews the benefits of maple sap as a bottled beverage while covering preservation and packaging methods, regulatory guidelines, and overview of commercial production procedures.Funding for project was made possible by a grant agreement from the U.S. Department of Agriculture (USDA) Agricultural Marketing Service. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA

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