Cell wall microdomains analysis in the quadrifids of Utricularia dichotoma

Carnivorous plants have fascinated botanists and ecologists with their various unusual adaptations in organ structure, physiology, and complex interactions with other organisms since the time of Charles Darwin. Species of the genus Utricularia (bladderworts, family Lentibulariaceae) are carnivorous plants that prey mainly on invertebrates using traps (bladders) of leaf origin. In the traps, there are glandular trichomes called quadrifids, which produce digestive enzymes and absorb the products of prey digestion. These quadrifids are unique due to their highly complex glandular cell structure; hence, they are an excellent model for studying the cell wall and its specialization. The main aim of the study was to investigate the presence and distribution of homogalacturonans (HGs) and hemicelluloses in the cell walls of trichome cells and especially in cell wall ingrowths in the quadrifid cells. The following antibodies were used against the wall components: anti-HGs (homogalacturonans) —JIM5 (low methylesterified HGs), JIM7 (highly esterified HGs), LM19 (low methylesterified HGs), CCRC-M38 (a fully de-esterified HG), LM5 (galactan); anti-hemicelluloses—LM25 (galactoxyloglucan; XXLLG, XXLG, XXXG modules of xyloglucans), LM15 (xyloglucan), CCRC-M138 (xylan), LM11 (heteroxylan); and anti-mannans: LM20 (heteromannan) and LM22 (heteromannan). The localization of the examined compounds was determined using immunohistochemistry techniques and immunogold labeling. In quadrifid cells, we found differences in the presence of the epitope detected by the LM5 antibody in the cell walls. In addition, cell wall ingrowths represented distinct microdomains of the cell wall in terms of the occurrence of wall components (they were methylesterified and demethylesterified homogalacturonan-poor). Hemicelluloses (galactoxyloglucan and xyloglucan) and arabinogalactans co-occur in cell wall ingrowths. Also, a part of the cell wall of the pedestal cell, which forms a Casparian strip, represented a distinct microdomain. We did not detect epitopes recognized by LM11, LM20 and LM22 antibodies. Our research shows that several cell wall microdomains occur in the cell walls of quadrifid cells. They differ depending on the presence and distribution of low methylesterified HGs, highly esterified HGs, fully de-esterified HGs, galactan (the epitope detected by the LM5 antibody), xyloglucan, galactoxyloglucan, and xylan (the epitope detected by the CCRC-M138 antibody)

Melting temperature hidden behind liquid–liquid phase transition in glycerol

Liquid–liquid phase transitions play a pivotal role in various scientific disciplines and technological applications, ranging from biology to materials science and geophysics. Understanding the behavior of materials undergoing these transitions provides valuable insights into complex systems and their dynamic properties. This review explores the implications of liquid–liquid phase transitions, particularly focusing on the transition between low-density liquid (LDL) and high-density liquid (HDL) phases. We investigate the thermodynamic, structural, and mechanistic aspects of these transitions, emphasizing their relevance in diverse fields. The creation of dynamic heterogeneities and critical fluctuations during liquid–liquid phase transitions is discussed, highlighting their role in shaping the phase behavior and dynamics of complex fluids. Experimental observations, including the use of dielectric spectroscopy and nonlinear methods, shed light on the intricate nature of these transitions. Our findings suggest a connection between liquid–liquid phase transitions and critical phenomena, with implications for understanding the supercooled state and phase behavior of hydrogen-bonded liquids such as glycerol. Overall, this review underscores the importance of interdisciplinary approaches in unraveling the complexities of liquid–liquid phase behavior and addressing fundamental questions

Cultural heritage and mobility from a multisensory perspective

Cultural Heritage and Mobility from a Multisensory Perspective bridges the gap between cultural heritage and mobility studies through the employment of theoretical and methodological multisensory perspectives. An interdisciplinary volume covering a broad range of empirical cases, this book focuses on the engagement with cultural heritage in the context of mobility. The book presents a grassroots perspective of individual heritage performances by mobile and moving actors, analyzing them with close attention to their embodied aspects: bodily experiences, sensory impressions, and the affect and emotions they evoke. As a result, the collection of case studies presented covers empirical, theoretical, and methodological accounts of the embodiment of heritage in the context of mobility on macro, meso, and micro levels, exploring heritage change and mobility from a multisensory perspective. Cultural Heritage and Mobility from a Multisensory Perspective is primarily targeted at scholars, students and practitioners working within and at the intersection of the fields of cultural heritage and mobility. It will also be of interest to those engaged in the study of tourism, migration and integration studies

Polish as a heritage language around the world : selected diaspora communities

Polish as a Heritage Language Around the World provides a timely insight into Polish diaspora communities around the world and their endeavours in heritage language maintenance and education. This edited collection depicts and analyses the unique challenges associated with the intergenerational transmission of Polish as a language that has not had high visibility and status in the surrounding society. Chapters within the volume examine how these circumstances impact the maintenance of the heritage language and affect the capacity to support biliteracy development among younger generations of speakers. Offering an overview of key concepts and theoretical issues, practical pedagogical guidance, and field‑advancing suggestions for further research, Polish as a Heritage Language Around the World will be of interest to researchers and instructors of Polish around the world, as well as those interested in second‑language acquisition and heritage language studies