Advancing Asset Tokenization in the European Union and Latvia : A Regulatory and Policy Perspective

Our study examines the regulatory challenges and opportunities of asset tokenization within the context of the European Union (EU), emphasizing the balance between technological innovation and investor protection in the digital economy. Focusing on 2023 EU Markets in Crypto-Assets Regulation and its application in Latvia, we utilize comparative legal and integrative literature review methodologies to explore how regulatory frameworks can enhance investor accessibility, liquidity, and transparency in digital transactions. Our findings emphasize the importance of strong legal frameworks in promoting economic growth and protecting investors, thereby contributing to a more inclusive financial ecosystem. By examining the regulatory landscape for distributed ledger technology, we provide insights into how regulations can balance innovation in asset management with the imperative of investor protection. We offer a broad analysis of the intersection between legal frameworks and technological advancements in Latvia, illustrating how diverse regulatory approaches can support both economic development and investor interests. Our research originality lies in its focus on the EU’s regulatory diversity, particularly in Latvia, and its implications for broader European and international regulatory environments. Our study contributes to ongoing discussions on optimizing regulatory strategies to facilitate secure and advantageous financial technologies, reflecting the diversity of legal and economic approaches across Europe.Peer reviewe

Human Defence Factors in Different Gestational Week Placenta : A Pilot Study

Publisher Copyright: © 2025 by the authors.Background: Numerous studies have shown the presence of multiple defence factors in placental tissue, although their role is partially understood; therefore, the aim of this study was to evaluate the expression of nuclear factor-kappa B (NF-κB); human beta-defensin 2, 3, and 4 (HBD-2,3,4); cathelicidine (LL-37); heat shock protein 60 (HSP60); and interleukin 10 (IL-10) in dissimilar gestational week placental tissue and display correlations between immunoreactive cells. Methods: A total of 15 human placental tissue samples were acquired from mothers with different gestational weeks: 28, 31, and 40. Routine staining and immunohistochemistry for the samples were executed. The evaluation of data was performed with semi-quantitative methods, and, for statistical analysis, the Kruskal–Wallis test was used. Spearman’s rank correlation was used for calculating correlations. Results: NF-κB, HBD- 2,3,4, HSP60, and IL-10 expression were discovered in every examined placental tissue cell type. LL-37 expression was found only in Hofbauer cells. A rise in expression with higher gestational weeks was noted in LL-37-positive Hofbauer cells (p = 0.03), HBD-3-positive cytotrophoblasts (p = 0.007), endothelial cells (p = 0.024), extraembryonic mesodermal cells (p = 0.004), and HBD-4-positive endothelial cells (p = 0.001). Numerous statistically significant moderate and strong positive correlations between defence factors were discovered. Conclusions: The persistence of Hofbauer cell accumulations underlines the growing significance of placental macrophages in placental protection. The expression of positive defence factors and a rise in expression in tissue protection factors (HBD-3, LL-37, HBD-4) in higher gestational weeks may indicate these factors as the most significant protectors of the placenta in ontogenetic aspects. The high number of statistically significant positive and negative correlations between positive cells show a strong network to sustain distressed placental growth and therefore pregnancy.Peer reviewe

Exploring Literacy and Knowledge Gaps and Disparities in Genetics and Oncogenomics Among Cancer Patients and the General Population : A Scoping Review

Publisher Copyright: © 2025 by the authors.Background: Genetic and genomic literacy is pivotal in empowering cancer patients and citizens to navigate the complexities of omics sciences, resolve misconceptions surrounding clinical research and genetic/genomic testing, and make informed decisions about their health. In a fast-evolving scenario where routine testing has become widespread in healthcare, this scoping review sought to pinpoint existing gaps in literacy and understanding among cancer patients and the general public regarding genetics and genomics. Methods: Adhering to the PRISMA framework, the review included 43 studies published between January 2018 and June 2024, which evaluated the understanding of genetics and genomics among cancer patients, caregivers, and citizens. Results: Although the selected studies had significant heterogeneity in populations and evaluation tools, our findings indicate inadequate literacy levels, with citizens displaying lower proficiency than cancer patients and caregivers. This review highlighted consistent knowledge gaps in understanding the genetic and genomic underpinnings of diseases, encompassing misconceptions about mutation types and inheritance patterns, limited awareness of available genetic testing options, and difficulties in interpreting test results. Ethical and privacy concerns and the psychological impact of genetic testing were also common, highlighting the imperative need for effective communication between healthcare providers and patients. Conclusions: Given the dynamic nature of genomic science, the review underscores the need for continuously evolving educational programs tailored to diverse populations. Our findings could guide the development of educational resources addressed explicitly to cancer patients, caregivers, and the lay public.Peer reviewe

A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia

Publisher Copyright: © 2025 The Author(s)Pneumonia stands as the primary cause of death among children under five, yet current diagnosis methods often result in inadequate or unnecessary treatments. Our research seeks to address this gap by identifying host transcriptomic biomarkers in the blood of children with definitive viral and bacterial pneumonia. We performed RNA sequencing on 192 prospectively collected whole blood samples, including 38 controls and 154 pneumonia cases, uncovering a 5-transcript signature (genes FAM20A, BAG3, TDRD9, MXRA7, and KLF14) that effectively distinguishes bacterial from viral pneumonia (area under the curve (AUC): 0.95 [0.88–1.00]). Initial validation using combined definitive and probable cases yielded an AUC of 0.87 [0.77–0.97], while full validation in a new prospective cohort of 32 patients achieved an AUC of 0.92 [0.83–1.00]. This robust signature holds significant potential to enhance diagnostics accuracy for pediatric pneumonia, reducing diagnostic delays and unnecessary treatments and potentially transforming clinical practice.Peer reviewe

Amygdala Nuclei Atrophy in Cognitive Impairment and Dementia : Insights from High-Resolution Magnetic Resonance Imaging

Publisher Copyright: © 2025 by the authors.Background and Objectives: Cognitive impairment affects memory, reasoning, and problem-solving, with early detection being critical for effective management. The amygdala, a key structure in emotional processing and memory, may play a pivotal role in detecting cognitive decline. This study examines differences in amygdala nuclei volumes in patients with varying levels of cognitive performance to evaluate its potential as a biomarker.  Material and methods: This cross-sectional study of 35 participants was conducted and classified into three groups: the normal (≥26), moderate (15-25), and low (≤14) cognitive performance groups based on the Montreal Cognitive Assessment (MoCA) scores. High-resolution magnetic resonance imaging at 3.0 T scanner was used to assess amygdala nuclei volumes.  Results: Significant amygdala atrophy was observed in multiple amygdala nuclei across cognitive performance groups, with more pronounced changes in the low-performance group. The right hemisphere nuclei, including the lateral and basal nuclei, showed more significant differences, indicating their sensitivity to cognitive decline.  Conclusions: This study highlights the potential of amygdala nuclei atrophy as a biomarker for cognitive impairment. Additional research with larger sample sizes and longitudinal designs is needed to confirm these findings and determine their diagnostic value.Peer reviewe

Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and is Involved in the Regulation of PSA Expression

Prostate-specific antigen remains a cornerstone biomarker for prostate cancer diagnosis and management. However, the molecular mechanisms regulating its expression, particularly through DNA methylation, are not fully understood. Here, we report a comprehensive analysis of allele-specific CpG and CCWGG methylation in the proximal PSA promoter across aggressive (PC3), indolent (LNCaP), benign (BPH1), and normal (HPrEpiC) prostate cell lines and provide insights into the unique methylation patterns associated with these states. Our findings reveal that PC3 cells, representing an aggressive PCa phenotype, exhibit complete biallelic methylation of the PSA promoter, leading to PSA gene silencing. Conversely, LNCaP cells display a fully unmethylated promoter with biallelic PSA expression. Interestingly, BPH1 cells display a monoallelic CG/CCWGG methylation pattern, yet fail to express PSA, suggesting imprinting defects or RNA decay mechanisms. Notably, acquisition of biallelic PSA promoter methylation status in PC3 was accompanied by upregulation of DNMT1, whereas unmethylated PSA promoter state in LNCaP was associated with downregulation of DNMT1. These findings highlight distinct methylation patterns in the PSA promoter that differentiate between aggressive, indolent, and benign prostate states. Translating this epigenetic insight into clinical diagnostics could enhance the precision of PSA-based diagnostics, addressing limitations such as false negatives in PSA testing for aggressive PCa. Further exploration of CCWGG methylation's role in imprinting and monoallelic expression is warranted, particularly in patient-derived samples.Peer reviewe

Simulācijā balstītas izglītības attīstības stratēģija medicīnas un veselības aprūpes izglītībā Latvijā 2025.–2027. gadam

Pamatojoties uz sadarbības memorandu “Par nacionālo simulācijā balstītas izglītības attīstības stratēģiju medicīnas un veselības aprūpes izglītībā”, kas tika noslēgts 2023. gada 7. decembrī starp Latvijas Republikas Veselības ministriju, Rīgas Stradiņa universitāti, Latvijas Universitāti, Latvijas Ārstu biedrību, Latvijas Jauno ārstu asociāciju, Latvijas Māsu asociāciju, Latvijas Ārstniecības personu profesionālo organizāciju savienību un Neatliekamās medicīniskās palīdzības dienestu, ir izstrādāts dokuments “Simulācijā balstītas izglītības attīstības stratēģija medicīnas un veselības aprūpes izglītībā Latvijā 2025.–2027. gadam” (turpmāk – Stratēģija). Ar šīs Stratēģijas izstrādi tiek atzīts simulācijā balstītas pieejas integrācijas svarīgums medicīnas un veselības aprūpes izglītībā, kā arī Stratēģijas izstrādes partneru apņemšanās sadarboties, iesaistot plašākas ieinteresēto personu grupas, lai uzlabotu gan esošo un nākotnes medicīnas un veselības aprūpes jomas profesionāļu izglītības kvalitāti, gan veselības aprūpes pakalpojumu kvalitāti, kas ietver gan pacientu, gan ārstniecības personu drošības aspektus. Šī Stratēģija ir ceļa karte, lai līdz 2027. gadam saskaņotā un koordinētā veidā tiktu veicināta simulācijā balstītas medicīnas un veselības aprūpes izglītības jomas integrācija visu līmeņu medicīnas un veselības aprūpes izglītībā, kā arī izveidots simulācijā balstīts medicīnas un veselības aprūpes izglītības jomas specifikai atbilstošs resursu un infrastruktūras pārvaldības modelis.Non peer reviewe

Concept Protocol for Developing a DAid® Smart Socks-Based Biofeedback System : Enhancing Injury Prevention in Football Through Real-Time Biomechanical Monitoring and Mixed Reality Feedback

Football players, particularly in youth leagues, face a high risk of lower limb injuries due to improper movement patterns. While programs like FIFA 11+ help reduce injuries, they lack real-time, personalized feedback for biomechanical correction. This concept protocol outlines the development of a DAid® smart socks-based biofeedback system that integrates biomechanical monitoring with mixed reality (MR) feedback to enhance injury prevention. The DAid® smart socks, equipped with pressure sensors and inertial measurement units (IMUs), track plantar pressure distribution and the center of pressure (COP). Real-time feedback is delivered via a Meta Quest 3 MR headset, enabling athletes to adjust movement patterns instantly. This protocol establishes a framework for evaluating the system’s feasibility and effectiveness in optimizing biomechanics and reducing injury risks. By combining wearable technology with MR-based feedback, this study advances injury prevention strategies, with potential applications in rehabilitation and performance training.Peer reviewe

Identification, Evaluation and Prioritization of Chemicals for National Human Biomonitoring Program : Insights from Latvia

Human biomonitoring (HBM) is a critical tool for assessing chemical exposure in populations and informing public health policies. This study aimed to prioritize chemical substances for the development of a national HBM program in Latvia, addressing the need for systematic evaluation of chemicals in the local context. Initially, 318 chemical substances were reviewed, of which 130 were shortlisted and assessed using an adapted Hanlon methodology. Substances were assessed based on their health significance, hazardous properties, exposure characteristics, national relevance, and public interest. The results identified 30 high-priority substances across various categories, providing a foundation for the HBM4LV program. This prioritization process highlighted the challenges of data gaps, resource limitations, and the need to balance national priorities with alignment to European frameworks. Despite addressing key methodological challenges, the study highlights the importance for ongoing refinement, robust data collection, and strengthened international collaboration to enhance the program’s scope and long-term sustainability. While the methodology addressed key challenges, further refinement and international collaboration are essential to enhance the program’s scope and sustainability.Peer reviewe

Cross-National Trends in Adolescents Psychological and Somatic Complaints Before and After the Onset of COVID-19 Pandemic

Publisher Copyright: © 2024 Society for Adolescent Health and MedicinePurpose: Building on research suggesting that the COVID-19 pandemic may have led to an exacerbation of deteriorating trends in mental health among adolescents, this paper examined trends in adolescents' psychological and somatic complaints across 35 countries from 2010 to 2022, and tested trends in sociodemographic inequalities in these outcomes between 2018 and 2022. Methods: Using data from 792,606 adolescents from 35 countries (51% girls; mean age = 13.5; standard deviation 1.6) across four Health Behaviour in School-aged Children surveys (2010, 2014, 2018, 2022), hierarchical multilevel models estimated cross-national trends in adolescent psychological and somatic complaints. We tested whether observed values in 2022 were in line with predicted values based on 2010–2018 linear trends. Finally, moderation effects of age, family affluence, and family structures on the outcomes were tested (2018–2022). Results: Both girls and boys showed substantially higher levels of psychological complaints in 2022 compared with the predicted values. For somatic complaints, higher levels than predicted in 2022 were observed only in girls. Moderation analyses revealed an increase from 2018 to 2022 in age gaps and a narrowing in the socioeconomic gap for both outcomes. Also, there was a widening gap between adolescents living with 2 parents and those living in a single parent household in 2022 compared to 2018. Discussion: Cross-national increases in adolescent psychological and somatic complaints were higher than expected in 2022, based on previous trends. Magnitudes of change varied across different sociodemographics groups, with implications for pre-existing mental health inequalities.Peer reviewe