HAL AMU

    NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1

    No full text
    International audiencePseudomonas aeruginosa is a highly adaptable opportunistic pathogen. It can infect vulnerable patients such as those with cystic fibrosis or hospitalized in intensive care units where it is responsible for both acute and chronic infection. The switch between these infections is controlled by a complex regulatory system involving the central GacS/GacA two-component system that activates the production of two small non-coding RNAs. GacS is a histidine kinase harboring one periplasmic detection domain, two inner-membrane helices and three H1/D1/H2 cytoplasmic domains. By detecting a yet unknown signal, the GacS histidine-kinase periplasmic detection domain (GacSp) is predicted to play a key role in activating the GacS/GacA pathway. Here, we present the chemical shift assignment of 96 % of backbone atoms (HN, N, C, Ca, Cb and Ha), 88 % aliphatic hydrogen atoms and 90 % of aliphatic carbon atoms of this domain. The NMR-chemical shift data, on the basis of Talos server secondary structure predictions, reveal that GacSp consists of 3 b-strands, 3 a-helices and a major loop devoid of secondary structures

    Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains

    No full text
    International audienceBeyond their hemostatic functions, platelets alter their inflammatory response according to the bacterial stimulus. Staphylococcus aureus is associated with exacerbated inflammation and thrombocytopenia, which is associated with poor prognosis during sepsis. Acetylsalicylic acid and statins prevent platelet aggregation and decrease the mortality rate during sepsis. Therefore, we assessed whether these two molecules could reduce in vitro platelet activation and the inflammatory response to S. aureus. Platelets were exposed to clinical strains of S. aureus in the presence or absence of acetylsalicylic acid or fluvastatin. Platelet activation, aggregation, and release of soluble sCD62P, sCD40 Ligand, RANTES and GROα were assessed. Platelet cell death was evaluated by analyzing the mitochondrial membrane potential, phosphatidylserine exposure, platelet microparticle release and caspase-3 activation. All S. aureus strains induced platelet activation but not aggregation and decreased the platelet count, the expression of cell death markers and the release of RANTES and GROα. Acetylsalicylic acid but not fluvastatin limited platelet activation and inflammatory factor release and restored the platelet count by protecting platelets from Staphylococcus-induced expression of cell death markers. This study demonstrates that acetylsalicylic acid limits S. aureus-induced effects on platelets by reducing cell death, revealing new strategies to reduce the platelet contribution to bacteremia-associated inflammation

    Microsatellite Primers in Parietaria judaica (Urticaceae) to Assess Genetic Diversity and Structure in Urban Landscapes

    No full text
    International audiencePremise of the study: Urbanization is one of the main factors contributing to loss of genetic diversity, as the resulting landscape fragmentation and habitat loss induce species isolation. However, studies of genetic structure and diversity in urbanized landscapes are still rare. We characterized microsatellite primers for Parietaria judaica to study this environment.Methods and Results: Eleven microsatellite loci from P. judaica, an urban plant, were isolated using shotgun pyrosequencing, and the simple sequence repeat (SSR) markers were screened in 20 individuals of P. judaica. The loci were tested on 166 individuals from three populations in different cities. The number of alleles ranged from two to 19, and expected and observed heterozygosity values ranged from 0.019 to 0.912 and 0.019 to 0.448, respectively. Conclusions: The markers amplified well in the species and will be useful for examining genetic diversity and population genetic structure in this urban plant

    Seasonal changes in the biochemical composition of the edible sea urchin Paracentrotus lividus (Echinodermata : Echinoidea) in a lagoonal environment

    No full text
    International audienceMonthly soluble and insoluble protein, carbohydrate, lipid and ash levels and energetic content were measured in tissues of Paracentrotus lividus adults living in a lagoonal environment over a 1-year period to increase our understanding of resource allocation to the different tissues. The biochemical composition of P. lividus individuals in this environment is similar to that observed in other environments. The gonad and gut contained a high level of proteins (soluble or insoluble), a lesser amount of lipids and, finally, a low level of carbohydrates. The main component of the test was ash, followed by protein levels. The lipid and carbohydrate levels in the test were very low. Seasonal variability in the biochemical composition of the different body parts was observed. In the gonad, an increase in the level of lipids was recorded concomitant to an increase in gonad weight. The lipids and carbohydrates. after an initial increase, decreased with the maturation of the gonad. The biochemical variations observed in the gut seem to be linked to both the reproductive cycle and the sea urchin's trophic activity. Variability in the test was low and irregular for all of the observed biochemical components. As P. lividus is an important member of the benthic community in the Urbinu lagoon, this echinoid represents an important store of materials and energy within this lagoonal environment

    Introductive note to the Special issue

    No full text
    International audienc

    La science étymologique de Mallarmé. Une lecture de "Victorieusement fui..."

    No full text
    International audienc

    Health-Related Quality of Life in Chronic HCV-Infected Patients Switching to Pegylated-Interferon-Free Regimens (ANRS CO20 CUPIC Cohort Study and SIRIUS Trial)

    No full text
    International audienceWe aimed to compare health-related quality of life (HRQL) during and after hepatitis C virus (HCV) treatment in patients receiving pegylated-interferon (PEG-IFN)-containing therapy (including boceprevir or telaprevir-ANRS CO20 CUPIC cohort) who subsequently switched to PEG-IFN-free regimens (sofosbuvir + ledipasvir with or without ribavirin [RBV]-SIRIUS trial)

    "La Ballade de Rikers Island: de l’affaire DSK à l’affaire Jauffret".

    No full text
    International audienc

    La tragédie spéculative de Racine, Hermann, mars 2014

    No full text
    International audienceRacine, le « Divin Sot », ne composa jamais d'ouvrages théoriques. Peut-on en conclure pour autant qu'il n'existe pas de théorie racinienne du genre tragique ? L'hypothèse de ce livre est que l'émule d'Euripide spéculait per actiones comme son grand modèle antique et ne cessa jamais d'écrire « la tragédie des tragédies ». Ses héros personnifient des styles et des idées ; ses intrigues sont des périphrases de débats spéculatifs et de conflits d'archétype ; le noeud, l'agôn et la conduite de ses tragédies successives allégorisent les querelles qui se succèdent au XVII e siècle autour du modèle tragique : querelle des cornéliens et des « doucereux », querelle des règles, querelle des anciens et des modernes, querelle des dévots ou « des Visionnaires ». D'Andromaque à Iphigénie et de Bérénice à Phèdre, Racine s'avance caché sous le masque de ses héros et sous le voile de ses intrigues. Ce sont ses argumentations que cet ouvrage déchiffre sous l'argument des tragédies
    HAL AMUis based in FR
    Do you manage HAL AMU? Access insider analytics, issue reports and manage access to outputs from your repository in the CORE Dashboard!