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Potential of Bacillus stercoris B.PNR2 to stimulate growth of rice and waxy corn under atrazine-contaminated soil
The presence of atrazine residue in agricultural soil may affect crop growth and the activity of plant growth-promoting bacteria. Therefore, this study investigated the impact of atrazine contamination on indole-3-acetic acid (IAA) production by Bacillus stercoris B.PNR2. Subsequently, the ability of B. stercoris B.PNR2 to stimulate the seedling growth of rice cultivars RD6 and Leum Pua, as well as the waxy corn cultivar Muang Tam, under atrazine contamination, was determined. The results showed that B. stercoris B.PNR2 produced IAA under various atrazine concentrations, and atrazine was not toxic to B. stercoris B.PNR2 cells. Atrazine at 20 mg/kg of soil did not affect the shoot and root dry weight of rice cultivars RD6 and Leum Pua, as well as the waxy corn cultivar Muang Tam grown in atrazine-contaminated soil without receiving a bacterial inoculum. The application of B. stercoris B.PNR2 did not stimulate the germination and growth of any of the plants used in this study. The application of B. stercoris B.PNR2 decreased the shoot and root dry weight of waxy corn grown under atrazine-contaminated soil. Additionally, the chlorophyll b and total chlorophyll content in rice cultivar RD6, grown under atrazine-contaminated soil, decreased to only 162.6 ± 4.2 and 616.0 ± 55.8 μg/g fresh weight, which was related to the increase in proline content to 343.6 ± 41.6 μg/g fresh weight. In conclusion, it can be stated that soaking seeds with B. stercoris B.PNR2 was not an appropriate means of inoculation to stimulate the growth of plants in this study
Ethnomedicines in the 21st century: challenges and opportunities in the contemporary world
The ethnomedicine industry inherently developed de novo in the evolutionary history of humanity to provide healthcare services. This industry has existed long before the currently preferred conventional medicine and by then was the only trusted means of healthcare to humanity. Ethnomedicine has evolved and diversified into specializations along ethnic groupings worldwide. Nevertheless, attitude towards ethnomedicine is usually polarized varying from contemptuous dismissal to romantic glorification. Rarely does one come across people with balanced views. The discussion is based on 9 issues: view of ethnoknowledge of health from the early days, discovery and development of ethnomedicines, specializations in ethnomedicines, challenges and limitations, terminologies that shape ethnomedicine industry, merits and demerits of ethnomedicines, existing opportunities and the way forward. The ethnomedicine industry, just like any other field of professionalism, was not without challenges and limitations that affected its realization of full potential. In this discussion, 24 challenges and limitations are outlined but not limited to:- lack of information on standards, safety and efficacy, capacity building of ethnopractitioners, colonialism, climate change, witchcraft, socio-politics, recognition of ethnosystems, research and biased documentation, emerging diseases and pathogens, market value-chain, in-situ and ex-situ conservation, anthropogenic activities, sources of ethnomedicines, legal frameworks, harvesting, preparation, administration and storage methods, gender issues, poor remembrance of ethnomedical knowledge, existence of quackery, sources of ethnomedicines, biopiracy and bioprospecting, lack of mechanisms for monitoring and evaluation of ethnomedicines, advancement in human evolution and civilization, biological evolution and invasive alien species, stigmatisation due to poor perceptions and attitudes about ethnomedicines and competitive supremacy amongst ethnopractitioners and conventional practitioners, have all not favoured the industry. However, the use of ethnomedicines has tremendously increased and continues to increase albeit the above challenges and limitations facing the industry. The challenges and limitations are broadly and critically discussed while outlining opportunities and giving away forward
Evaluation of antimicrobial and anticancer efficacy of silver nanoparticles phytofabricated by Nyctanthes arbor-tristis L. leaf extract
Nyctanthes arbor-tristis L. (Oleaceae), commonly known as harsingar, is a plant with potential medicinal properties. The plant was used in traditional folk medicine and as a pharmacological activity. The present study reports a rapid and eco-accommodating technique to synthesize silver nanoparticles AgNPs with low cost and with no need to heat, using aqueous extracts of N. arbor-tristis L. Phytochemical analysis was conducted to detect the existence of alkaloids, flavonoids, saturated sugar, saponins, glycosides, steroids, tannins, terpenoids, and proteins in the plant. A color change observed in the test confirmed the synthesis of AgNPs. SEM, XRD, EDX, FTIR, and UV – Visible spectrophotometer (DLS) were used to characterize the synthesized AgNPs. The antibacterial potential of AgNPs was further tested against different bacterial strains such as Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus and Klebsiella pneumonia displayed by finding the maximum zone of inhibition observed against S. typhi of 24.6 mm, S. aureus 21.6±0.57 mm, P. aeruginosa 19 mm, K. pneumonia 17 mm and E. coli 15.3±0.57 mm. Furthermore, the synthesized AgNPs were also exhibited as anticancer properties against MDA-231 cell line Human Breast cancer cell line which was determined dose dependent manner and their concentration of 2.5 to 30 μg/mL respectively, and noted the inhibitory range at 30 μg/mL of its concentration, which was further tested in high inhibitory effect on their leaf extract high concentration in cytotoxic, bactericidal experiments
Cytotoxicity effect and antioxidant potential of 5-Hydroxymethyl Furfural (5-HMF) analogues-An advance approach
Rivea hypocrateriformis (Desr.) Choisy is a profound medicinal belongs to the family Convolvulaceae. Natural products are considered as an alternative source for a positive approach to the drug design and drug discovery. R. hypocrateriformis is becoming the most important natural source to produce diverse phytometabolites with varying biochemical activities. Therefore, in the proposed study, we utilizing R. hypocrateriformis for isolating the 5-hydroxymethyl-2-furfural (5-HMF) and characterized it by different scientifically approved spectroscopic techniques namely 1HNMR, 13C NMR, FTIR and mass spectroscopy respectively. As a part of this study, the synthesis of chemical analogues has been achieved by coupling 5-HMF with quinoline derivatives and it was also studied for their antioxidant and anticancer potentials. The results demonstrated that amongst the test compounds, 3d and 3b have shown significant free radical scavenging assay followed by 3e and 3a with a maximum inhibitory effect, 76.69 %, 75.90 %, 67.60 % and 56.07 % respectively at 50 μg/mL. The anticancer activity studied through SRB assay showed that, compound 3a was effective at low concentration (10 μg/mL) against the Colo-205 cell line. This study demonstrated the applicability of R. hypocrateriformis against the cytotoxicity and antioxidant potential of 5-HMF. It can further be utilized by the researcher and pharmaceutical industry to design a potential drug candidate to treat cellular toxicity
Effect of nano urea on growth and yield of scented geranium (Pelargonium graveolens L. Herit): Effect of nano urea on scented geranium
Nanotechnology holds promise for optimizing nutrient delivery in agriculture. This study examines the impact of nano urea, a nanoscale urea formulation, on scented geranium (Pelargonium graveolens) growth and yield. A randomized controlled trial compared nano urea with traditional urea on various growth and yield parameters. Nano urea treatment, T7-15 kg N neem coated urea + 0.5 ml l-1 nano urea improved the growth and yield of scented geranium. Plants sprayed with nano urea had maximum growth (plant height, number of leaves per plant, number of branches per plant, plant spread), yield (fresh herbage yield (1.53 and 1.26 kg per plant, 28.76 and 25.56 kg per net plot and 14.27 and 12.68 t per hectare) and essential oil yield per hectare (14.70 and 13.11 kg) in both the main as well as ratoon crops respectively
Regulation of cell proliferation and tumor suppressor roles of microRNA 329-3p of the MAP kinase pathway in cervical squamous carcinoma
Cervical squamous cell carcinoma is observed as the second major cause of mortality worldwide. A highly conserved mitogen-activated protein kinase (MAPK) signaling pathway occurs in a wide range of cellular processes which includes differentiation, proliferation, migration, senescence, and apoptosis. MAPK pathway can be activated by various extracellular signals, capable of generating responses as per the cell type. Thus, alteration of the EGFR receptor in this particular pathway leads to the condition of cancer due to abnormal activation of receptor tyrosine kinases. The characteristic features of microRNA (miRNA) which are endogenous, single-stranded, small non-coding RNA for their role in RNA silencing and post-transitional regulation of gene expression have been studied over the years. The miRNA functions by base pairing with the complementary sequences within the mRNA molecule. One such miRNA, miR-329-3p has a critical tumor suppressor role in the MAPK pathway, however, is least understood. Therefore, miRNA could be considered as a potential biomarker for diagnosis, prognosis, and therapeutic purposes and brought out to its fullest use to mankind
Unveiling black pepper diversity: A morphological characterization of genotypes in Sylhet, Bangladesh: Black pepper diversity in Sylhet, Bangladesh
Black pepper (Piper nigrum L.) is a valuable spice and despite having suitable climatic conditions, Bangladesh relies on imports to meet the demand. There is some homestead production of black pepper, but information available on the genotypes grown in Bangladesh is scanty. Here, we characterized black pepper genotypes in the Sylhet region for potential domestic production. Healthy, disease-free cuttings of eleven genotypes (G-1 to G-11) were collected and grown in a nursery condition for characterization. Diverse traits were observed for lamina shape (ovate, ovate-lanceolate, ovate-elliptic, and cordate), leaf tip (acute, accuminate, and caudate), leaf base (cordate and rounded), shoot tip color (green, light green, yellowish-green, purple, and dark purple), and leaf margin (wavy and even). Quantitative parameters revealed substantial variation in leaf length (6.8 to 11.24 cm), leaf width (3.66 to 6.24 cm), leaf area (26.8 to 66.6 cm2), petiole length (2.3 to 5.0 cm), internode length (1.93 to 6.2 cm), and leaf dry matter content (19.6 to 30.4%). Notably, G-8 exhibited superior values for leaf dimensions, area, petiole and internode lengths, along with relatively higher leaf dry matter content (23.9%). This study provides a baseline for selecting and breeding high-yielding black pepper varieties in Bangladesh, potentially boosting domestic production
Determination of irrigation requirement for tomato using FAO-CROPWAT model in the Arid Region
Food security presents a significant challenge in arid regions, exacerbated by water scarcity due to climate change. Tomato is one of the top agricultural crops around the world, in which it is needed to investigate its irrigation water requirements for each region, especially in the arid regions. This work mainly aims to estimate the irrigation water requirements and irrigation schedules for cultivating tomato in Ras Al Khaimah (RAK), United Arab Emirates (UAE). This would be done by utilizing the FAO-CROPWAT 8.0 Model and CLIMWAT 2.0. The findings of this research support efficient water use by utilizing the advanced modeling tools. The outcomes will inform and enhance irrigation water management practices for farmers, farm owners, and policymakers, contributing significantly to sustainable development goals and the resilience of agricultural systems in water-limited environments
Potential of Chromolaena odorata, Ipomoea carnea and Eichhornia crassipes as green manures on soil fertility index and rice production on vertisols
The organic farming system is an agricultural activity which aims to preserve soil fertility and create sustainable farming. The objective of this research is to evaluate the effects of the application of green manure on soil fertility index and rice production. The research was carried out in Weru District, Sukoharjo Regency, using an experiment method with a Randomized Complete Block Design (RCBD). The research included 10 treatments: T0 = Control, T1 = NPK fertilizer 0.2 ton/ha, T2 = rice straw 10 ton/ha, T3 = Chromolaena odorata 10 ton/ha, T4 = Ipomoea carnea 10 ton/ha, T5 = Eichhornia crassipes 10 ton/ha, T6 = NPK 0.1 ton/ha + rice straw 5 ton/ha, T7 = NPK 0.1 ton/ha + C. odorata 5 ton/ha, T8 = NPK 0.1 ton/ha + I. carnea 5 ton/ha, T9 = NPK 0.1 ton/h + E. crassipes 5 ton/ha, with three repetitions. The soil characteristics tested were pH, total N, available P, exchangeable K, exchangeable Ca, exchangeable Mg, Cation Exchange Capacity (CEC), Base Saturation (BS), and Soil Organic Carbon (SOC). The results show that C. odorata, I. carnea, and E. crassipes have potential as green manures, due to their high N content, of 3.38%, 3.54%, and 2.95% andthe ability to increase soil fertility and rice production. The soil fertility index increased from high to very high, neither within single applications nor combined with NPK fertilizer. The highest rice production was obtained with the use of C. odorata, which increased harvest dry weight by 2.24 ton/ha (67.67%) or an increase of 2.1 ton/ha (75%) in milled dry weight, followed by I. carnea and E. crassipes. There was a correlation between soil fertility index and rice production, shown by the higher SFI, the harvest dry weight and milled dry weight increases
Calibration and validation of APSIM-Wheat Model in Mediterranean areas
The Agricultural Production Systems sIMulator-Wheat (APSIM-Wheat) model is one of the most widely used agricultural models. It is a powerful simulator that has been successfully calibrated and tested for many locations in the world, especially in Western Australia (WA). However, there is a noticeable lack of a standard guide for realizing the calibration validation of APSIM-Wheat that could be applied in areas with a Mediterranean climate similar to that of WA. Therefore, this study aims to examine crop simulations reported in published articles and to provide a detailed description of input data and statistical assessment, which represent the two main components of the calibration-validation protocols. The PRISMA (PREFERRED Reporting Items for Systematic Reviews and Meta-Analyses) method was used to identify and select relevant papers for this review. Following the analysis of 31 calibration protocols extracted from selected eligible articles, it was found that regardless of the objective of using APSIM-Wheat, the same category of data is required for calibration. As far as meteorological data is concerned, the information essential to this study was daily maximum and minimum air temperatures, rainfall (mm), and solar radiation. In the case of soil data, information about the texture and hydraulic characteristics, especially PAWC, DUL and LL was required. Regarding agricultural management data, this pertains to cultivated crops, Nitrogen fertilization (rate and time of application) and sowing (date and density). For the statistical evaluation, it was observed that 90 percent of studies analyzed in this review revealed the use of RMSE